medlitГигиена и санитарияHygiene and Sanitation0016-99002412-0650Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0016-9900-2020-99-12-1346-1352medlit-1131Research ArticleГИГИЕНА ОКРУЖАЮЩЕЙ СРЕДЫENVIRONMENTAL HYGIENEОбщетоксическое и кардиовазотоксическое действие наночастиц оксида кадмияGeneral toxic and cardiovascular toxic impact of cadmium oxide nanoparticleshttps://orcid.org/0000-0002-0927-4062КлиноваСветлана ВладиславовнаKlinovaSvetlana V.

Науч. сотр. лаб. промышленной токсикологии отдела токсикологии и биопрофилактики ФБУН «Екатеринбургский медицинский – научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, Екатеринбург.

e-mail: klinova.svetlana@gmail.com

Researcher of Department of the Toxicology and Bioprophylaxis, Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, Yekaterinburg, 620014, Russian Federation.

e-mail: klinova.svetlana@gmail.com

klinova.svetlana@gmail.comМинигалиеваИ. А.MinigalievaIlzira A.noemail@neicon.ruhttps://orcid.org/0000-0001-8750-9624КацнельсонБ. А.KatsnelsonBoris A.noemail@neicon.ruhttps://orcid.org/0000-0001-8580-403XСоловьеваС. Н.SolovyevaSvetlana N.noemail@neicon.ruhttps://orcid.org/0000-0002-1442-6737ПриваловаЛ. И.PrivalovaLarisa I.noemail@neicon.ruhttps://orcid.org/0000-0002-6475-7753ГурвичВ. Б.GurvichVladimir B.noemail@neicon.ruhttps://orcid.org/0000-0003-2677-0479РябоваЮ. В.RyabovaIuliia V.noemail@neicon.ruhttps://orcid.org/0000-0002-2018-5386ЧернышовИ. Н.ChernyshovIvan N.noemail@neicon.ruhttps://orcid.org/0000-0002-5872-2001БушуеваТ. В.BushuevaTatiana V.noemail@neicon.ruhttps://orcid.org/0000-0002-2726-9259СахаутдиноваР. Р.SakhautdinovaRenata R.noemail@neicon.ruhttps://orcid.org/0000-0002-6970-7798ШурВ. Я.ShurVladimir Ya.noemail@neicon.ruhttps://orcid.org/0000-0002-2574-7472ШишкинаЕ. В.ShishkinaEkaterina V.noemail@neicon.ruhttps://orcid.org/0000-0002-1743-7642СутунковаМ. П.SutunkovaMarina P.noemail@neicon.ruФБУН «Екатеринбургский медицинский - научный центр профилактики и охраны здоровья рабочих промпредприятий» РоспотребнадзораYekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial WorkersУЦКП «Современные нанотехнологии» ИЕНиМ УрФУ им. первого Президента России Б.Н. ЕльцинаModern nanotechnologies, Ural Federal University202020012021991213461352Copyright © Клинова С.В., Минигалиева И.А., Кацнельсон Б.А., Соловьева С.Н., Привалова Л.И., Гурвич В.Б., Рябова Ю.В., Чернышов И.Н., Бушуева Т.В., Сахаутдинова Р.Р., Шур В.Я., Шишкина Е.В., Сутункова М.П., 20212021Клинова С.В., Минигалиева И.А., Кацнельсон Б.А., Соловьева С.Н., Привалова Л.И., Гурвич В.Б., Рябова Ю.В., Чернышов И.Н., Бушуева Т.В., Сахаутдинова Р.Р., Шур В.Я., Шишкина Е.В., Сутункова М.П.Klinova S.V., Minigalieva I.A., Katsnelson B.A., Solovyeva S.N., Privalova L.I., Gurvich V.B., Ryabova I.V., Chernyshov I.N., Bushueva T.V., Sakhautdinova R.R., Shur V.Y., Shishkina E.V., Sutunkova M.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rjhas.ru/jour/article/view/1131Введение

Введение. Предприятия цветной металлургии являются одним из основных источником поступления наноразмерных частиц кадмия в воздух рабочих помещений и атмосферу населённых мест.

Материал и методы

Материал и методы. Внутрибрюшинные введения суспензии наночастиц оксида кадмия (НЧ CdO) проводили 3,5-месячным аутбредным крысам-самцам 3 раза в неделю на протяжении 6 нед (всего 18 введений) в разовой дозе 0,25 мг/кг массы тела. По завершении экспозиции состояние организма крыс оценивалось по большому числу (свыше пятидесяти) общепризнанных критериев токсического действия (включая биохимические и цитоморфометрические). Статистический анализ полученных результатов проводился с использованием t-критерия Стьюдента.

Результаты

Результаты. Гематотоксическое действие НЧ CdO проявилось снижением содержания гемоглобина, ростом числа ретикулоцитов, эозинофилов и моноцитов. НЧ CdO влияли на порфириновый обмен (рост δ-аминолевуленовой кислоты в моче). Токсическое поражение печени характеризовалось увеличением массы органа, снижением содержания альбуминов, А/Г-индекса, а также повышением уровня γ-глутамилтранспептидазы и липопротеидов высокой плотности в сыворотке крови. Отмечено усиление оксидативного стресса (снижение активности каталазы и содержания церулоплазмина).

Показано снижение содержания эндотелина-1, следствием чего может являться обнаруженное снижение показателей артериального давления (статистически значимое для среднего АД).

Заключение

Заключение. При субхронической экспозиции к НЧ CdO была получена интоксикация умеренной степени тяжести. Она характеризовалась массовыми гематологическими, биохимическими и цитоморфометрическими изменениями. Обнаружен слабовыраженный, но явный кардиовазотоксический эффект кадмия.

Introduction

Introduction. Non-ferrous smelters are one of the critical nanoscale cadmium particles sources in the workplace and ambient air.

Materials and methods

Materials and methods. The research was performed to evaluate the subchronic toxicity of cadmium oxide nanoparticles (CdO-NPs) in white outbred rats. Male outbred 3.5-month old rats received intraperitoneal injections of CdO-NPs 3 times a week for six weeks (18 in total) in doses of 0.25 mg/kg body mass. After the end of an exposure, there were rated more than 50 indices of universally accepted toxicity criteria (including biochemical and cytomorphometric). Student’s t-test was used for statistical analysis.

Results

Results. The hematotoxic effects of CdO-NPs were revealed by a decrease in the hemoglobin content, an increase in the number of reticulocytes, eosinophils, and monocytes. CdO NPs influenced porphyrin metabolism (an increase of δ-aminolevulinic acid in the urine). Liver toxicity resulted in an increase in organ mass and a decrease in albumin content and A/G index. Besides, there was observed a rise in γ-glutamyl transpeptidase and high-density lipoproteins in the blood serum. Oxidative stress level increased (decrease in catalase action and ceruloplasmin content). Endothelin-1 decreased. It may result from an observed decrease in blood pressure indices (statistically significant for mean B.P.).

Conclusion

Conclusion. The intoxication of moderate severity was retrieved at the end of the subchronic exposure to cadmium oxide nanoparticles. It characterized mass, hematological, biochemical, and cytomophometric changes. There was found mild but evident cardiovascular toxicity of cadmium oxide nanoparticles.

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The authors declare that there are no conflicts of interest present.