medlitГигиена и санитарияHygiene and Sanitation0016-99002412-0650Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0016-9900-2021-100-11-1333-1338medlit-1857Research ArticleМЕТОДЫ ГИГИЕНИЧЕСКИХ ИССЛЕДОВАНИЙMETHODS OF HYGIENIC AND EXPERIMENTAL INVESTIGATIONSЦитомный анализ: современный универсальный инструмент медико-биологических и эколого-гигиенических исследований (обзор литературы). Часть 2Cytomic analysis: a modern universal tool for biomedical and ecological and hygienic research (literature review). Part 2https://orcid.org/0000-0002-5039-8980КривцоваЕлена КонстантиновнаKrivtsovaElena K.

Науч. сотр. отд. профилактической токсикологии и медико-биологических исследований НИИ ЭЧ и ГОС им. А.Н. Сысина ФГБУ «ЦСП» ФМБА России, 119121, Москва.

e-mail: EKrivcova@cspmz.ru

MD, researcher of the Department of Preventive Toxicology and Biomedical Research in the Centre for Strategic Planning of the Federal Medical Biological Agency, Moscow, 119121, Russian Federation.

e-mail: EKrivcova@cspmz.ru

ekrivcova@cspmz.ruhttps://orcid.org/0000-0002-2262-6800ИнгельФ. И.IngelFaina I.noemail@neicon.ruhttps://orcid.org/0000-0002-3619-3858АхальцеваЛ. В.AkhaltsevaLyudmila V.noemail@neicon.ruФГБУ «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Федерального медико-биологического агентстваРоссияCentre for Strategic Planning and Management of Biomedical Health Risks of the Federal Medical Biological AgencyRussian Federation2021061220211001113331338Copyright © Кривцова Е.К., Ингель Ф.И., Ахальцева Л.В., 20212021Кривцова Е.К., Ингель Ф.И., Ахальцева Л.В.Krivtsova E.K., Ingel F.I., Akhaltseva L.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rjhas.ru/jour/article/view/1857

Во второй части обзора приведены примеры использования цитомного анализа как на лимфоцитах периферической крови, так и на эпителиоцитах человека при изучении профессиональных рисков, а также воздействия на геном таких вредных привычек, как употребление алкоголя и табака. Большое внимание уделено применению цитомного анализа в эколого-гигиенических исследованиях при изучении влияния на население как природных, так и антропогенных, особенно производственных, факторов. Подробно изложен метод цитомного анализа на первичной культуре лимфоцитов периферической крови человека в условиях цитокинетического блока (CBMN-cyt тест) в его современном состоянии, а также кратко отражена история его развития. Разобран также метод цитомного анализа на буккальных эпителиоцитах человека (BMN-cyt). В обзоре приведены микрофотографии нарушений клетки, являющихся показателями этих тестов. При подборе литературы использованы базы данных PubMed, Web of Science, ResearchGate, Scopus, eLibrary.

Участие авторов

Участие авторов: Кривцова Е.К. — поиск источников литературы, анализ и интерпретация данных литературы, написание текста;Ингель Ф.И. — концепция и дизайн исследования, поиск источников литературы;Ахальцева Л.В. — поиск источников литературы, анализ и интерпретация данных литературы.Все соавторы — утверждение окончательного варианта статьи, ответственность за целостность всех частей статьи.

Конфликт интересов

Конфликт интересов. Авторы декларируют отсутствие явных и потенциальных конфликтов интересов в связи с публикацией данной статьи.

Финансирование

Финансирование. Исследование не имело спонсорской поддержки.

Поступила

Поступила: 27.04.2021 / Принята к печати: 28.09.2021 / Опубликована: 30.11.2021

In the second part of the review, examples of cytome analysis on both peripheral blood lymphocytes and human buccal epiteliocytes are given in the study of occupational risks and investigation of genome instabilities induced by alcohol and tobacco use. Much attention is paid to the cytome analysis application in environmental and hygienic research and the studies directed to evaluating natural and anthropogenic load to the human population, especially industrial, factors. The method of cytome analysis on primary culture of human peripheral blood lymphocytes cultivated with a cytokinetic block (CBMN-cyt test) in its current state is described in detail, and the history of its development is briefly reflected. The method of cytome analysis on human buccal epiteliocytes (BMN-cyt) is also analyzed. The review contains photomicrographs of genetic cell damages that are markers in micronuclei (MN) tests. We used PubMed, Web of Science, ResearchGate, Scopus, eLibrary databases as the sources of literature.

Contribution

Contribution: Krivtsova E.K. — search of literature sources, analysis and interpretation of literature data, writing a text;Ingel F.I. — the concept and design of the study, a search of literature sources;Akhaltseva L.V. — search of literature sources, analysis and interpretation of literature data.All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement

Acknowledgement. The study had no sponsorship.

Received

Received: April 27, 2021 / Accepted: September 28, 2021 / Published: October 31, 2021

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The authors declare that there are no conflicts of interest present.