medlitГигиена и санитарияHygiene and Sanitation0016-99002412-0650Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0016-9900-2020-99-2-200-205medlit-209Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL INVESTIGATIONSБаланс глутатионзависимых процессов в альвеолярных макрофагах крыс линии Wistar при воздействии твёрдых взвешенных частиц атмосферного воздухаBalance of glutathione-related processes in alveolar macrophages under exposure to suspended particulate matter of atmospheric air in of Wistar ratshttps://orcid.org/0000-0002-1009-9011ВиткинаТ. И.VitkinaT. I.noemail@neicon.ruhttps://orcid.org/0000-0001-7582-343XБарсковаЛюдмила СергеевнаBarskovaLyudmila S.

Аспирант, младший научный сотрудник лаборатории медицинской экологии и рекреационных ресурсов Владивостокского филиала ДНЦ ФПД – НИИМКВЛ.

e-mail: pretty_people_2016@mail.ru

MD, postgraduate student, Junior Researcher of the Laboratory of Medical Ecology and Recreational Resources of the Vladivostok branch of the Far Eastern Scientific Center of Physiology and Pathology of Respiration – Institute of Medical Climatology and Rehabilitative Treatment, Vladivostok, 690105, Russian Federation.

e-mail: pretty_people_2016@mail.ru

pretty_people_2016@mail.ruhttps://orcid.org/0000-0002-0498-9085ЗюмченкоН. Е.ZyumchenkoN. E.noemail@neicon.ruhttps://orcid.org/0000-0002-5788-6290ТокмаковаН. П.TokmakovaN. P.noemail@neicon.ruhttps://orcid.org/0000-0002-6413-9840ГвозденкоТ. А.GvozdenkoT. A.noemail@neicon.ruhttps://orcid.org/0000-0002-4873-2281ГолохвастК. С.GolokhvastK. S.noemail@neicon.ruВладивостокский филиал Федерального государственного бюджетного научного учреждения «Дальневосточный научный центр физиологии и патологии дыхания» - Научно-исследовательский институт медицинской климатологии и восстановительного леченияVladivostok Branch of the Far Eastern Scientific Center of Physiology and Pathology of Respiration - Institute of Medical Climatology and Rehabilitative TreatmentФедеральное государственное автономное образовательное учреждение высшего образования «Дальневосточный федеральный университет»Far Eastern Federal UniversityВладивостокский филиал Федерального государственного бюджетного научного учреждения «Дальневосточный научный центр физиологии и патологии дыхания» - Научно-исследовательский институт медицинской климатологии и восстановительного лечения; Федеральное государственное автономное образовательное учреждение высшего образования «Дальневосточный федеральный университет»Vladivostok Branch of the Far Eastern Scientific Center of Physiology and Pathology of Respiration - Institute of Medical Climatology and Rehabilitative Treatment; Far Eastern Federal University202023032020992200205Copyright © Виткина Т.И., Барскова Л.С., Зюмченко Н.Е., Токмакова Н.П., Гвозденко Т.А., Голохваст К.С., 20202020Виткина Т.И., Барскова Л.С., Зюмченко Н.Е., Токмакова Н.П., Гвозденко Т.А., Голохваст К.С.Vitkina T.I., Barskova L.S., Zyumchenko N.E., Tokmakova N.P., Gvozdenko T.A., Golokhvast K.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rjhas.ru/jour/article/view/209Введение

Введение. Твёрдые взвешенные частицы (ТВЧ) атмосферного воздуха оказывают непосредственное воздействие на органы дыхания человека. Одной из характеристик ТВЧ, определяющих их патогенность, является дисперсность. В ответ на воздействие альвеолярные макрофаги (АМ) вырабатывают активные формы кислорода, что может приводить к развитию окислительного стресса.

Цель – оценить вклад глутатионового звена антиоксидантной системы в защиту АМ от окислительного стресса, индуцированного воздействием ТВЧ атмосферного воздуха районов г. Владивостока с различной техногенной нагрузкой.

Материал и методы

Материал и методы. Выделяли АМ бронхоальвеолярного лаважа 17 крыс линии Вистар с последующим проведением нагрузочных тестов модельными взвесями (МВ) в течение 2 сут. МВ идентичны по составу ТВЧ атмосферному воздуху районов г. Владивостока с незначительной (МВ № 1) и высокой (МВ № 2) техногенной нагрузкой. МВ № 1 содержала 22% частиц c диаметром менее 10 мкм. В МВ № 2 частицы с диаметром менее 10 мкм составили 70%. Определяли содержание малонового диальдегида, глутатиона и общую антиоксидантную активность в культуре клеток и культуральной жидкости.

Результаты

Результаты. Установлено, что увеличение доли высокодисперсных частиц ведёт к усилению пероксидации липидов в АМ и компенсаторному возрастанию антиоксидантной активности. Повышение уровня окисленного глутатиона в культуре клеток свидетельствует об интенсификации процессов детоксикации гидропероксидов АМ. Снижение экзоцитоза восстановленного глутатиона поддерживает внутриклеточные антиоксидантные процессы.

Заключение

Заключение. При увеличении в атмосферном воздухе доли мелкодисперсных частиц окислительно-восстановительный баланс АМ может смещаться в сторону развития окислительного стресса, способствуя формированию и прогрессированию патологических нарушений.

Introduction

Introduction. Air suspended particulate matter (SPM) directly influence on the human respiratory system. Dispersion is one of the characteristics of SPM determining their pathogenicity. Alveolar macrophages (AMs) produce reactive oxygen species in response to an exposure that may lead to oxidative stress.

The aim of the study

The aim of the study. To assess the contribution of the glutathione antioxidant system to the protection of AMs from oxidative stress induced by air SPMs in Vladivostok’s districts with various man-made loads.

Material and methods

Material and methods. AMs were isolated from bronchoalveolar lavage of 17 Wistar rats. AMs were exposed by model suspensions (MS) for 2 days. MSs were identical air composition of Vladivostok’s districts with insignificant (MS № 1) and high (MS № 2) technogenic load. MS № 1 contained 22% of particles with a diameter of less than 10 µm. MS № 2 contained 70% of particles with a diameter smaller than 10 μm. The levels of malondialdehyde, glutathione and total antioxidant activity in cell culture and culture fluid were determined.

Results

Results. The gain in the proportion of fine and ultrafine particles in MS № 2 has been established to lead to an increase in lipid peroxidation in AMs and a compensatory elevation in the antioxidant activity. The elevation of oxidized glutathione concentration in cell culture indicates the intensification of hydroperoxide detoxification by AMs. The reduction of reduced glutathione exocytosis supports intracellular antioxidant processes.

Conclusion

Conclusion. An increase in the fraction of fine and ultrafine particles in the air appears to shift AM redox balance towards oxidative stress, contributing to the formation and the progression of pathological disorders.

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The authors declare that there are no conflicts of interest present.