medlitГигиена и санитарияHygiene and Sanitation0016-99002412-0650Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0016-9900-2019-98-3-332-338medlit-297Research ArticleМЕТОДЫ ГИГИЕНИЧЕСКИХ ИССЛЕДОВАНИЙMETHODS OF HYGIENIC AND EXPERIMENTAL INVESTIGATIONSВлияние перорально вводимых одностенных углеродных нанотрубок на интегральные и биохимические показатели крыс WistarInfluence of single-walled carbon nanotubes ingestion by rats on their integral and biochemical indiceshttps://orcid.org/0000-0002-0015-8735ШипелинВ. А.ShipelinV. A.noemail@neicon.ruhttps://orcid.org/0000-0003-1373-4436ШумаковаА. А.ShumakovaA. A.noemail@neicon.ruhttps://orcid.org/0000-0001-6622-5251СотоХ. С.SotoKh. S.noemail@neicon.ruСелифановА. В.SelifanovA. V.noemail@neicon.ruСидороваЮ. С.SidorovaYu. S.noemail@neicon.ruМасютинА. Г.MasyutinA. G.noemail@neicon.ruhttps://orcid.org/0000-0002-6492-9068ЧерновА. И.ChernovA. I.noemail@neicon.ruhttps://orcid.org/0000-0002-3671-6508ГмошинскийИван ВсеволодовичGmoshinskiIvan V.

Доктор биол. наук, вед. науч. сотр. лаборатории пищевой токсикологии и оценки безопасности нанотехнологий ФГБУН «ФИЦ питания и биотехнологии», 109240, г. Москва.

e-mail: gmosh@ion.ru 

MD, Ph.D., DSci., D.Sc, Ph.D. in Biology, leading researcher of the Department of Food Toxicology and Evaluation of Nanotechnology Safety, Federal Research Centre for Nutrition and Biotechnology, Moscow, 109240, Russian Federation.

e-mail: gmosh@ion.ru

gmosh@ion.ruhttps://orcid.org/0000-0002-5340-9649ХотимченкоС. А.KhotimchenkoS. A.noemail@neicon.ruФГБУН «ФИЦ питания и биотехнологии», 109240, МоскваFederal Research Centre for Nutrition and BiotechnologyМосковский государственный университет им. М.В. Ломоносова, 119991, МоскваLomonosov Moscow State UniversityФГБУН «Институт общей физики им. А.М. Прохорова» РАНProkhorov General Physics Institute of the Russian Academy of Sciences109240, Москва; ФГАОУ ВО «Первый МГМУ им. И.М. Сеченова» Минздрава России (Сеченовский Университет)Federal Research Centre for Nutrition and Biotechnology; I.M. Sechenov First Moscow State Medical University (Sechenov University)201914102020983332338Copyright © Шипелин В.А., Шумакова А.А., Сото Х.С., Селифанов А.В., Сидорова Ю.С., Масютин А.Г., Чернов А.И., Гмошинский И.В., Хотимченко С.А., 20202020Шипелин В.А., Шумакова А.А., Сото Х.С., Селифанов А.В., Сидорова Ю.С., Масютин А.Г., Чернов А.И., Гмошинский И.В., Хотимченко С.А.Shipelin V.A., Shumakova A.A., Soto K.S., Selifanov A.V., Sidorova Y.S., Masyutin A.G., Chernov A.I., Gmoshinski I.V., Khotimchenko S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rjhas.ru/jour/article/view/297Введение

Введение. Одностенные углеродные нанотрубки (ОСУНТ) могут воздействовать на организм человека в результате их использования в упаковочных материалах, стимуляторах роста с/х растений и перспективных агрохимикатах. Целью работы явилось изучение влияния ОСУНТ на крыс при пероральном введении в течение 92 суток.

Материал и методы

Материал и методы. ОСУНТ диспергировали ультразвуком в воде с 1% неионогенного ПАВ Tween-20. По данным динамического рассеяния света, электронной микроскопии и комбинационного светорассеяния в образце присутствовали как свободные, так и частично агрегированные ОСУНТ. Эксперимент проведён на пяти группах растущих самцов крыс Wistar. Дисперсию ОСУНТ добавляли к питьевой воде в дозе 0 (контроль); 0,01; 0,1; 1,0 и 10,0 мг/кг массы тела (м. т) для групп 1–5 соответсвенно. Изучали уровень тревожности и когнитивную функцию в тесте условного рефлекса пассивного избегания (УРПИ); определяли массу внутренних органов, проницаемость стенки тонкой кишки для макромолекул овальбумина, биохимические показатели сыворотки крови, активность глутатионпероксидазы, содержание небелковых тиолов в печени, экскрецию с мочой 8-гидрокси-2’-дезоксигуанозина (8-oxo-G) и селена. 

Результаты

Результаты. Под действием ОСУНТ наблюдали достоверные изменения содержания в сыворотке крови общего белка, мочевой кислоты, триглицеридов, холестерина, активности аспартатаминотрансферазы, относительной массы печени, а также снижение проницаемости стенки кишки для макромолекул белка. Отмечались повышение экскреции 8-oxo-G, снижение активности глутатионпероксидазы, содержания глутатиона в печени, показателя обеспеченности селеном. Большинство из вышеперечисленных эффектов в большей степени проявлялись при минимальных и средних дозах ОСУНТ, чем при максимальных. Влияния ОСУНТ на поведенческие реакции выявлено не было.

Заключение

Заключение. Полученные данные свидетельствуют о наличии у ОСУНТ признаков токсического действия в дозе 0,1 мг/кг массы тела в сутки и менее. 

Introduction

Introduction. Single-walled carbon nanotubes (SWCNTs) can affect the human body as a result of their use in packaging materials, growth stimulators of agricultural plants and promising agrochemicals. 

The aim of the study was to investigate the effect of SWCNT on integral and biochemical indices on the model of oral administration to rats in a subacute experiment with duration of 92 days.

Material and methods

Material and methods. SWCNT were dispersed by ultrasound in water with 1% by weight of the non-ionic surfactant Tween 20. According to the data of dynamic light scattering, electron microscopy, and Raman light scattering the sample containing both free and partially aggregated SWCNTs. The experiment was carried out on 5 groups of growing male Wistar rats. The SWCNT dispersion was added to the drinking water at doses (control); of 0.01; 0.1; 1.0 and 10 mg/kg body weight in groups from the 1st to the 5th group. We studied the level of anxiety and cognitive function in the test of the “Conditional Reflex of Passive Avoidance” (CRPA); the mass of internal organs, the permeability of the small intestine wall for ovalbumin macromolecules, the biochemical parameters of blood serum, the activity of glutathione peroxidase, the content of non-protein thiols in the liver, urinary excretion of 8-hydroxy-2’-deoxyguanosine (8-oxo-G) and selenium.

Results

Results. As a result of SWCNTs consumption there were found significant changes in the content of total protein, uric acid, triglycerides, cholesterol in the blood serum, the increased activity of aspartate aminotransferase, the relative mass of the liver, and decreased the permeability of the intestinal wall for macromolecules of the protein. There have been shifts in individual antioxidant protection indices, including increased 8-oxo-G excretion, decreased glutathione peroxidase activity, glutathione content in the liver, and selenium availability. Most of the above effects did not show a dose dependence of the nanomaterial and were more pronounced at minimal and medium doses of SWCNTs than at maximum doses. The effect of SWCNTs on the behavioral responses of animals was not revealed. 

Conclusion

Conclusion. Therefore, SWCNT show signs of toxic effects at a dose of 0.1 mg/kg body weight in a day or less.

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The authors declare that there are no conflicts of interest present.