medlitГигиена и санитарияHygiene and Sanitation0016-99002412-0650Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0016-9900-2024-103-6-527-532aaxnzomedlit-4127Research ArticleГИГИЕНА ОКРУЖАЮЩЕЙ СРЕДЫENVIRONMENTAL HYGIENEИсследование и сравнительная оценка бионакопления и негативных эффектов при хроническом ингаляционном воздействии нано- и микрочастиц оксида алюминия в экспериментеResearch and comparative assessment of bioaccumulation and negative effects during chronic inhalation exposure to nano- and microparticles of aluminum oxide in an experimenthttps://orcid.org/0000-0002-7226-7682СтепанковМарк СергеевичStepankovMark S.

Мл. науч. сотр., аспирант ФБУН «ФНЦ МПТ УРЗН», 614045, Пермь, Россия

e-mail: stepankov@fcrisk.ru

MD, junior researcher, postgraduate student of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Perm, 614045, Russian Federation

e-mail: stepankov@fcrisk.ru

stepankov@fcrisk.ruhttps://orcid.org/0000-0002-8013-9613ЗемляноваМарина АлександровнаZemlyanovaMarina A.

Доктор мед. наук, доцент, зав. отд. биохимических и цитогенетических методов диагностики ФБУН «ФНЦ МПТ УРЗН», 614045, Пермь, Россия

e-mail: zem@fcrisk.ru

MD, PhD, DSci., assistant professor, head of Department of Biochemical and Cytogenetic Diagnostics of Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Perm, 614045, Russian Federation; Perm State National Research University, Perm, 614990, Russian Federation; Perm National Research Polytechnic University, Perm, 614990, Russian Federation

e-mail: zem@fcrisk.ru

zem@fcrisk.ruФБУН «Федеральный научный центр медико-профилактических технологий управления рисками здоровью населения»РоссияFederal Scientific Center for Medical and Preventive Health Risk Management TechnologiesRussian FederationФБУН «Федеральный научный центр медико-профилактических технологий управления рисками здоровью населения»; ФГБОУ ВО «Пермский государственный национальный исследовательский университет»; ФГБОУ ВО «Пермский национальный исследовательский политехнический университет»РоссияFederal Scientific Center for Medical and Preventive Health Risk Management Technologies; Perm State National Research University; Perm National Research Polytechnic UniversityRussian Federation2024170720241036527532Copyright © Степанков М.С., Землянова М.А., 20242024Степанков М.С., Землянова М.А.Stepankov M.S., Zemlyanova M.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rjhas.ru/jour/article/view/4127Введение

Введение. В связи с загрязнением атмосферного воздуха наночастицами Al2O3 (НЧ), обладающими отличными от микрочастиц (МЧ) физическими свойствами, актуальным является выявление особенностей негативного воздействия НЧ Al2O3 при длительном ингаляционном поступлении.

Цель работы — исследование и сравнительная оценка бионакопления и негативных эффектов, ассоциированных с хроническим ингаляционным воздействием нано- и микрочастиц Al2O3, в эксперименте.

Материалы и методы

Материалы и методы. Исследованы физические свойства НЧ Al2O3 в сравнении с МЧ. На крысах линии Wistar определены отличительные особенности бионакопления и негативные эффекты, ассоциированные с хронической ингаляционной экспозицией НЧ Al2O3 в концентрации 0,017 мг/м3 в течение 180 сут.

Результаты

Результаты. НЧ Al2O3 обладают меньшим размером, большей удельной площадью поверхности и суммарным объёмом пор, что способствует их лучшей по сравнению с МЧ проникающей активности через защитные барьеры. Благодаря этому в соответствующих органах биораспределения НЧ обладают большей степенью бионакопления. Воздействие НЧ вызывает окислительно-восстановительный дисбаланс, цитолиз, гепатотоксический эффект, нарушение процессов возбуждения и торможения нервной системы, тромбоцитоз. При экспозиции МЧ менее выражены окислительно-восстановительный дисбаланс, цитолиз и нарушение нейропередачи возбуждения. НЧ вызывают патоморфологические изменения в виде воспаления и нарушения циркуляции крови в тканях лёгких, нарушения циркуляции крови в головном мозге и печени. При экспозиции МЧ установлено лишь развитие воспалительного процесса в лёгких.

Ограничения исследования

Ограничения исследования. Исследование выполнено только при хронической ингаляционной экспозиции НЧ и МЧ Al2O3 на крысах линии Wistar.

Заключение

Заключение. НЧ Al2O3 обладают более выраженным бионакоплением, большим спектром и степенью проявления негативных эффектов в сравнении с МЧ. Полученные результаты целесообразно применять для повышения эффективности научного обоснования рекомендаций, направленных на предотвращение и минимизацию негативных эффектов со стороны здоровья, ассоциированных с хроническим ингаляционным воздействием НЧ Al2O3.

Соблюдение этических стандартов. Исследование выполнено в соответствии с Европейской конвенцией по защите позвоночных животных, используемых для экспериментальных или в иных научных целях (ETS № 123) и требованиями этического комитета ФНЦ Медико-профилактических технологий управления рисками здоровью населения (протокол № 3 от 27.03.2018 г.).

Участие авторов

Участие авторов:Землянова М.А. — концепция и дизайн исследования, обработка материала, редактирование;Степанков М.С. — сбор материала, обработка материала, написание текста.Все соавторы — утверждение окончательного варианта статьи, ответственность за целостность всех частей статьи.

Конфликт интересов

Конфликт интересов. Авторы декларируют отсутствие явных и потенциальных конфликтов интересов в связи с публикацией данной статьи.

Финансирование

Финансирование. Исследование выполнено за счёт федерального бюджета.

Поступила

Поступила: 03.04.2024 / Принята к печати: 19.06.2024 / Опубликована: 17.07.2024

Introduction

Introduction. In connection with atmospheric air pollution by Al2O3 nanoparticles (NPs), which have physical properties different from microparticles (MPs), it is relevant to identify the key features of the negative impact of Al2O3 NPs during long-term inhalation intake.

The aim of the study

The aim of the study. Research and comparative assessment of bioaccumulation and negative effects associated with Al2O3 nano- and microparticles chronic inhalation intake the body in an experiment.

Materials and methods

Materials and methods. The physical properties of Al2O3 NPs were studied in comparison with MPs. Using Wistar rats, the distinctive features of bioaccumulation and negative effects associated with chronic inhalation exposure to Al2O3 NPs at a concentration of 0.017 mg/m3 for 180 days were determined.

Results

Results. Al2O3 NPs have a smaller size, larger specific surface area and total pore volume, which contributes to their greater penetrating activity through protective barriers compared to MPs. Due to this, with the same range of biodistribution organs, NPs have a higher degree of bioaccumulation. Exposure to NPs causes redox imbalance, cytolysis, hepatotoxic effect, disruption of the processes of excitation and inhibition of the nervous system, and thrombocytosis. When exposed to MP, less pronounced redox imbalance, cytolysis, and disruption of the process of neurotransmission of excitation were noted. NPs cause pathomorphological changes in the form of inflammation and impaired blood circulation in lung tissues, impaired blood circulation in the brain and liver. When exposed to MP, only the development of an inflammatory process in the lungs was established.

Limitations

Limitations. The study was carried out only with chronic inhalation exposure to Al2O3 NPs and MPs on Wistar rats.

Conclusion

Conclusion. Al2O3 NPs have more pronounced bioaccumulation, a larger spectrum and degree of manifestation of negative effects in comparison with MPs. It is advisable to use the obtained results to improve the effectiveness of scientific substantiation of recommendations aimed at preventing and minimizing negative health effects associated with chronic inhalation exposure to Al2O3 NPs.

Compliance with ethical standards: the study was carried out in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental or other Scientific Purposes (ETS No. 123) and the requirements of the Ethics Committee of the Federal Scientific Center for Medical and Preventive Technologies for Public Health Risk Management (protocol No. 3 of 27.03.2018).

Contribution

Contribution:Zemlyanova M.A. — the concept and design of the study, processing of the material, editing, statistical processing;Stepankov M.S. — collection of material, processing of material, writing the text.All co-authors — approval of the final version of the article, responsibility for the integrity of all parts of the article.

Conflict of interest

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement

Acknowledgement. The study was carried out at the expense of the federal budget.

Received

Received: April 3, 2024 / Accepted: June 19, 2024 / Published: July 17, 2024

 

 

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The authors declare that there are no conflicts of interest present.