medlitГигиена и санитарияHygiene and Sanitation0016-99002412-0650Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0016-9900-2019-98-12-1437-1443medlit-478Research ArticleЗДОРОВЬЕ НАСЕЛЕНИЯPOPULATION HEALTHСодержание антител к антигенам Helicobacter pylori и маркёров воспалительной реакции в сыворотках крови жителей МосквыLevels of h pylori antibodies and inflammatory markers in blood serum samples of Moscow residentshttps://orcid.org/0000-0003-0170-3085ХрипачЛюдмила ВасильевнаKhripachLudmila V.

Доктор биол. наук, зав. лаб. биохимических и молекулярно-генетических методов исследования ФГБУ «ЦСП» МЗ РФ, 119991, Москва.

e-mail: lkhripach@mail.ru

MD, Ph.D., Dsci., head of the laboratory of biochemical and molecular genetics methods, Centre for Strategic Planning, Russian Ministry of Health, Moscow, 119992, Russian Federation.

e-mail: lkhripach@mail.ru

lkhripach@mail.ruhttps://orcid.org/0000-0001-5279-5018КнязеваТ. Д.KnyazevaT. D.noemail@neicon.ruhttps://orcid.org/0000-0001-9339-9310ЖелезнякЕ. В.ZheleznyakE. V.noemail@neicon.ruhttps://orcid.org/0000-0002-1628-199XГерманС. В.GermanS. V.noemail@neicon.ruhttps://orcid.org/0000-0002-3902-7521ЗыковаИ. Е.ZykovaI. E.noemail@neicon.ruhttps://orcid.org/0000-0003-4772-9686ЗагайноваА. В.ZagainovaA. V.noemail@neicon.ruhttps://orcid.org/0000-0002-7942-8004ЮдинС. М.YudinS. M.noemail@neicon.ruФедеральное государственное бюджетное учреждение «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Министерства здравоохранения Российской ФедерацииCentre for Strategic Planning, Russian Ministry of Health201914102020981214371443Copyright © Хрипач Л.В., Князева Т.Д., Железняк Е.В., Герман С.В., Зыкова И.Е., Загайнова А.В., Юдин С.М., 20202020Хрипач Л.В., Князева Т.Д., Железняк Е.В., Герман С.В., Зыкова И.Е., Загайнова А.В., Юдин С.М.Khripach L.V., Knyazeva T.D., Zheleznyak E.V., German S.V., Zykova I.E., Zagainova A.V., Yudin S.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rjhas.ru/jour/article/view/478Введение

Введение. Helicobacter pylori (Hр) является одним из факторов развития гастрита, язвенной болезни и рака желудка и в то же время фактором, препятствующим возникновению астмы, диабета 2-го типа и ожирения. Целью данного исследования является изучение возможной связи между содержанием антител к Нр и маркёров воспалительной реакции – ИЛ-6, ИЛ-8 и малонового диальдегида (МДА) – в сыворотках крови взрослых жителей Москвы.

Материал и методы

Материал и методы. В пробах сыворотки крови обследуемых лиц (252 человека обоего пола трудоспособного возраста) измеряли содержание антител к лизатному антигену Нр, рекомбинантному антигену CagA, и интерлейкинов ИЛ-6, ИЛ-8 с использованием коммерческих тест-наборов ИФА. Содержание МДА оценивали по реакции с тиобарбитуровой кислотой.

Результаты

Результаты. Стандартным корреляционным анализом не найдено достоверных связей между содержанием в сыворотках антител к изучавшимся антигенам Нр и содержанием в них ИЛ-6, ИЛ-8 и МДА. На двумерных графиках рассеяния значений анти-CagA/ИЛ-8 (но не ИЛ-6 и МДА) в серопозитивной области видны две параллельные ветви экспериментальных точек, соответствующие по расположению двум максимумам на бимодальном распределении содержания антител к CagA. Полученные результаты соответствуют общепринятым представлениям о характерной для CagA опережающей индукции ИЛ-8 и свидетельствуют о наличии в популяции человека представителей двух генотипов с примерно двукратным различием по способности синтезировать антитела к CagA, но с одинаковой реактивностью оси «CagA – ИЛ-8». Сходство амплитуд CagA-зависимого и CagA-независимого (из других органов) выброса ИЛ-8 в кровь, при отсутствии признаков окислительного стресса, в равной степени позволяет интерпретировать наблюдаемые эффекты как ранние патологические или как реальные симбиотические процессы.

Заключение

Заключение. Вклад популяционных исследований в изучение характера взаимодействия между Нр и организмом человека увеличится, если использовать количественное определение циркулирующих антител и не ограничиваться применением стандартных статистических тестов.

Introduction

Introduction. Helicobacter pylori (Hр) is considered usually as one of the factors in the development of gastritis, peptic ulcer, and gastric cancer, and at the same time as protection from asthma, diabetes type II, and obesity. This study was carried out with a goal to evaluate possible linkage between the levels of circulating anti-Hp antibodies and inflammatory markers - IL-6, IL-8 and malonic dialdehyde (MDA) - in cross-sectional study of Moscow adults.

Material and Methods

Material and Methods. Serum samples of Moscow working-aged residents (both gender, N=252) were used for evaluation of antibodies to lysate Hp antigen/recombinant CagA and cytokines IL-6, IL-8 with corresponding ELISA kits. MDA was determined by reaction with thiobarbituric acid.

Results

Results. Standard correlation analysis didn’t reveal any significant association between the levels of circulating antibodies against the applied commercial antigens and the levels of IL-6, IL-8 and MDA. Nevertheless, two parallel branches of the experimental points in seropositive area of anti-CagA/IL-8 scatterplot (but not for IL-6 and MDA variables) were clearly seen and correspond to two maximums at bimodal anti-CagA distribution. The data obtained are consistent with typical for CagA outstrip in IL-8 induction and testify the existence of two human genotypes having ~2-fold difference in antibody response but the same reactivity of CagA - IL-8 axis. Since CagA-positive IL-8 levels are of one order with CagA-negative ones (from another organs) and signs of concomitant oxidative stress were not revealed, the data obtained can be interpreted equally as early pathologic or actual symbiotic events.

Conclusion

Conclusion. Quantitative assessment of circulating anti-Hp antibodies, together with more detail mathematical analysis, will increase contributions of population studies to investigation of equilibrium between Hp and human organisms.

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The authors declare that there are no conflicts of interest present.