medlitГигиена и санитарияHygiene and Sanitation0016-99002412-0650Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0016-9900-2025-104-4-503-509rmxyecmedlit-4832Research ArticleМЕТОДЫ ГИГИЕНИЧЕСКИХ ИССЛЕДОВАНИЙMETHODS OF HYGIENIC AND EXPERIMENTAL INVESTIGATIONSИдентификация плесневых грибов методом MALDI-TOF: подходы и перспективы (обзор литературы)Identification of filamentous fungi with MALDI-TOF: methods and perspectives (literature review)https://orcid.org/0000-0003-3152-4862КурбатоваИрина ВалентиновнаKurbatovaIrina V.

Канд. мед. наук, ст. науч. сотр. лаб. микробиологии и паразитологии ФГБУ «ЦСП» ФМБА России, 119121, Москва, Россия

e-mail: IKurbatova@cspfmba.ru

PhD (Biology), senior researcher, Laboratory of Microbiology and Parasitology, Centre for Strategic Planning of the Federal medical and biological agency, Moscow, 119121, Russian Federation

e-mail: IKurbatova@cspfmba.ru 

IKurbatova@cspfmba.ruhttps://orcid.org/0000-0003-3554-7690РакитинаДарья ВикторовнаRakitinaDaria V.

Канд. биол. наук, ст. науч. сотр. лаб. микробиологии и паразитологии ФГБУ «ЦСП» ФМБА России, 119121, Москва, Россия

e-mail: Rakitina@cspmz.ru

PhD (Biology), senior researcher, Laboratory of Microbiology and Parasitology, Centre for Strategic Planning of the Federal medical and biological agency, Moscow, 119121, Russian Federation

e-mail: Rakitina@cspmz.ru

Rakitina@cspmz.ruФГБУ «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Федерального медико-биологического агентстваРоссияCentre for Strategic Planning of the Federal medical and biological agencyRussian Federation2025300420251044503509Copyright © Курбатова И.В., Ракитина Д.В., 20252025Курбатова И.В., Ракитина Д.В.Kurbatova I.V., Rakitina D.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rjhas.ru/jour/article/view/4832

В обзоре систематизированы представленные в литературе сведения об адаптации метода MALDI-TOF к идентификации плесневых грибов. Масс-спектрометрическая идентификация бактерий методом MALDI-TOF является «золотым стандартом» в современной лабораторной диагностике. Точность, скорость, простота и невысокая стоимость одного анализа обеспечивают всё более широкое распространение этого метода. Однако применение технологии MALDI-TOF к другим микроорганизмам, в том числе плесневым грибам, затруднено, поскольку прочная стенка клеток грибного мицелия препятствует клеточному лизису и ионизации белков, а разнообразие клеток в культуре (гифы, споры, органы размножения) приводит к разнообразию ионов белков и усложняет получение единого воспроизводимого спектра для изолята. Для преодоления этих трудностей применяются различные методы культивирования, подготовки образцов, получения и обработки спектров. На рынке присутствуют готовые решения в этой области в виде коммерческих систем, однако и они требуют доработки.

Участие авторов

Участие авторов: Курбатова И.В. – концепция обзора, редактирование; Ракитина Д.В. – подбор литературы, написание текста. Все соавторы – утверждение окончательного варианта статьи и ответственность за целостность всех её частей.

Конфликт интересов

Конфликт интересов. Авторы декларируют отсутствие явных и потенциальных конфликтов интересов в связи с публикацией данной статьи.

Финансирование

Финансирование. Исследование проведено в рамках НИР по теме «Разработка унифицированных методов, включающих отбор 8807 проб, для осуществления определения микробиологического и паразитологического загрязнения сточных вод» (шифр «Сточные воды»).

Поступила

Поступила: 27.08.2024 / Принята к печати: 03.12.2024 / Опубликована: 30.04.2025

This review addresses various attempts of adaptation of MALDI-TOF method for identification of filamentous fungi. This method has become a golden standard in laboratory diagnostics. Accuracy, simplicity, and low cost per analysis promote making it a widespread technology. However, attempts of applying it to filamentous fungi face many problems. Solid cell wall of fungi mycelium is an obstacle to cell lysis and protein ionization. Heterogeneity of cells in culture – old and young hyphs, conidia etc, provides variety in ion patterns and prevents forming single reproducible spectra for the analyzed isolate. These issues are addressed by various optimizations in methods of cultivation, sampling, protein extraction, spectra recording, and data analysis. Commercial systems exist, offering ready solutions of the problem; however, these systems also require adjustments to be effective in the clinical laboratory practice.

Contribution of the authors: Kurbatova I.V. – concept and design of the study, editing; Rakitina D.V. – literature search, writing. All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version

Conflict of interest

Conflict of interest. The authors declare no conflict of interest.

Acknowledgment

Acknowledgment. The research was carried out within the framework of the research work “Development of unified methods, including sampling, for the determination of microbiological and parasitological contamination of wastewater” (code “Wastewater”).

Received

Received: August 27, 2024 / Accepted: December 3, 2024 / Published: April 30, 2025

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The authors declare that there are no conflicts of interest present.