medlitГигиена и санитарияHygiene and Sanitation0016-99002412-0650Federal Scientific Center of Hygiene named after F.F. Erisman10.47470/0016-9900-2018-97-6-501-504medlit-577Research ArticleГИГИЕНА ОКРУЖАЮЩЕЙ СРЕДЫENVIRONMENTAL HYGIENEКонтроль загрязнения атмосферного воздуха при применении пестицидов на основе триазолинтионаControl of the pollution of atmospheric air under the use of pesticides on the basis of triazolinonehttps://orcid.org/0000-0003-3324-5090ГречинаМ. С.GrechinaM. S.noemail@neicon.ruhttps://orcid.org/0000-0003-1342-1536ИвченковаА. А.IvchenkovaA. A.noemail@neicon.ruhttps://orcid.org/0000-0001-8278-6382ФёдороваНаталия ЕвгеньевнаFedorovaNataliia E.

Д-р биол. наук, зав. отделом аналитических методов контроля ФБУН «Федеральный научный центр гигиены им. Ф.Ф. Эрисмана» Роспотребнадзора.

e-mail: analyt1@yandex.ru

MD, Ph.D., DSci., Head of the Department of analytical control methods of the F.F. Erisman Federal Scientific Center of Hygiene of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, 141014, Mytischi, Russian Federation.

e-mail: analyt1@yandex.ru

analyt1@yandex.ruФБУН «Федеральный научный центр гигиены им. Ф.Ф. Эрисмана» РоспотребнадзораF.F. Erisman Federal Scientific Center of Hygiene of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing201820102020976501504Copyright © Гречина М.С., Ивченкова А.А., Фёдорова Н.Е., 20202020Гречина М.С., Ивченкова А.А., Фёдорова Н.Е.Grechina M.S., Ivchenkova A.A., Fedorova N.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rjhas.ru/jour/article/view/577Введение

Введение. В статье представлено аналитическое решение по контролю в атмосферном воздухе действующего вещества — пестицида химического класса триазолинтиона — протиоконазола, являющегося системным фунгицидом нового поколения, обладающего защитным, искореняющим и лечебным действием. 

Материал и методы

Материал и методы. Метод основан на высокоэффективной жидкостной хроматографии с ультрафиолетовым детектором (длина волны детектирования — 213 нм), включает отбор проб воздушной среды на бумажные фильтры «синяя лента» со скоростью аспирации 5 дм3/мин. Экстракцию протиоконазола с фильтров выполняют ацетонитрилом. Для концентрирования экстракта с фильтров используют приём твердофазной экстракции с применением патронов на основе октадецилсилана. Отмечено, что концентрирование не может быть выполнено напрямую без предварительного разбавления аликвоты экстракта водой в объёмном соотношении 1 : 9. Из-за особенностей данного действующего вещества, его склонности к деградации для стабилизации получаемых водных растворов использована аминокислота цистеин. 

Результаты

Результаты. Линейность градуировочной характеристики подтверждена в диапазоне концентраций 0,05 – 0,5 мкг/см3 (коэффициент корреляции более 0,999). Нижний предел количественного определения протиоконазола в воздушной среде составляет 0,0025 мг/м3 при аспирации 80 дм3 воздуха, что в 8 раз ниже установленной величины ОБУВ протиоконазола в атмосферном воздухе (0,02 мг/м3). Суммарная погрешность измерения не превышает 16%. Разработанный метод апробирован в натурных исследованиях при определении экспозиционных уровней протиоконазола в пробах атмосферного воздуха в пределах санитарного разрыва, отобранных при штанговом опрыскивании полевых культур, протравливании и высеве семян зерновых, сои, кукурузы, протравливании клубней картофеля с одновременной высадкой, авиаобработке полевых культур (подсолнечника).

Introduction

Introduction. The analytical decision on control of active ingredient of pesticides of a chemical class of a triazolintione — the prothioconazole considered as the system fungicide of the new generation possessing protective, eradicating and medical action in atmospheric air is presented in the article.

Material and methods

Material and methods. The method is based on HPLC with the UV-detector (wavelength of detecting of 213 nm), includes a sampling of the air environment on paper filters “blue film” with a speed of aspiration of 5 l/min. The extraction of a prothioconazole from filters is carried out an acetonitrile. For the concentrating of the extract from filters, there was used the reception of SPE with the application of cartridges on the basis of C18. It is noted that concentrating cannot be executed directly without preliminary dilution of an aliquot of the extract by water in a volume ratio of 1:9. Because of features of this active ingredient, his tendency to degradation for stabilization of the received water solutions amino acid cysteine is used.

Results

Results. The linearity of the calibration characteristic is confirmed in the range of concentration of 0.05 – 0.5 µ/ml (correlation coefficient more than 0.999). The lower limit of quantitation of a prothioconazole in the air environment amounts to 0.0025 mg/m3 under the aspiration of 80 L of air that is 8 times lower than the established border level of a prothioconazole in atmospheric air (0.02 mg/m3). The total error of the measurement does not exceed 16%. The developed method was applied for the determination of prothioconazole’ exposure levels in natural conditions in samples of atmospheric air within a sanitary gap taken of processing of spraying of field cultures, preliminary processing of seeds of grain, soy, corn, and potatoes, aircraft processing of field cultures (sunflower).

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The authors declare that there are no conflicts of interest present.