Influence of ApoE gene polymorphism on development of obesity and blood biochemical parameters in coal mine workers

Introduction. The prevalence of obesity is becoming more relevant every year. Individuals with increased body weight show alterations in various biochemical parameters including lipid profile. One of the genes involved in the regulation of lipid metabolism is the APOE gene.

The aim of the work. To study the influence of ApoE gene on biochemistry parameters and BMI level in coal mine workers.

Materials and Methods. Data of one hundred thirty four coal mine workers were included. Venous blood was collected from all participants for DNA and serum isolation. Further genotyping was carried out for two polymorphic variants of ApoE gene (rs7412 and rs429358). Along with this, blood biochemical parameters were evaluated.

Results. While studying the effect of two polymorphic variants on BMI in subjects, a significant association was noted among carriers of E2/E3 and E3/E3 genotypes. Among biochemical indices, had an effect on cholesterol, folic acid, homocysteine, total calcium, low density lipoproteins, alkaline phosphatase, γ-glutamyltransferase, and fibrinogen.

Limitations. The results obtained characterize the situation at a specific production site (coal mine) in a particular region (Russia, Western Siberia). In the future, the reproducibility of the results of our study will be tested on other, larger samples with a wide range of genetic variants related to lipid metabolism and eating behaviour.

Conclusion. The results obtained in this study may be useful for further work in the formation of certain risk groups and preventive measures.

Compliance with ethical standards. The study was approved by the decision of the Ethical Committee of Kemerovo State University (protocol No. 11 of 13 June 2024). All participants gave informed voluntary written consent to participate in the study.

Contribution:
Yakovleva A.A. – material collection and data processing, text writing;
Minina V.I. – research concept and design, editing;
Soboleva O.A. – Material collection and data processing;
Borisova O.I. – material collection and data processing;
Bulatova O.V. – editing;
Prosekov A.Y. – research concept and design.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

Received: June 25, 2024 / Accepted: December 3, 2024 / Published: March 31, 2025

1. Timofeev Yu.S., Dzhioeva O.N., Drapkina O.M. Circulating biological markers of obesity: towards a systems approach. Kardiovaskulyarnaya terapiya i profilaktika. 2014; 22(4): 85–93. https://doi.org/10.15829/1728-8800-2023-3551 https://elibrary.ru/qfortp (in Russian)

2. Polyanina D.D., Bereza I.A., Amromina A.M., Shaikhova D.R., Astakhova S.G., Sutunkova M.P., et al. Polymorphism of the APOE gene as a risk factor of obesity in workers exposed to occupational hazards at ferrous metallurgy enterprises. Analiz riska zdorov’yu. 2023; (2): 155–9. https://doi.org/10.21668/health.risk/2023.2.15 https://elibrary.ru/pclbmi (in Russian)

3. Alferova V.I., Mustafina S.V. The prevalence of obesity in the adult population of the Russian Federation (literature review). Ozhirenie i metabolizm. 2022; 19(1): 96–105. https://doi.org/10.14341/omet12809 https://elibrary.ru/ecocvf (in Russian)

4. Shenkova N.N., Veselovskaya N.G., Chumakova G.A., Osipova E.S., Gritsenko O.V., Ott A.V. Risk prediction for subclinical atherosclerotic lesion of brachiocephalic arteries in obese women. Rossiiskii kardiologicheskii zhurnal. 2017; 22(4): 54–60. https://doi.org/10.15829/1560-4071-2017-4-54-60 https://elibrary.ru/ymhtrz (in Russian)

5. Filimonov E.S., Korotenko O.Yu., Ulanova E.V. Cardiorespiratory disorders in abdominal obesity in workers of coal mining enterprises in the south of Kuzbass. Meditsina truda i promyshlennaya ekologiya. 2021; 61(3): 168–172. https://doi.org/10.31089/1026-9428-2021-61-3-168-172 https://elibrary.ru/nxczxp (in Russian)

6. Klop B., Elte J.W., Cabezas M.C. Dyslipidemia in obesity: mechanisms and potential targets. Nutrients. 2013; 5(4): 1218–40. https://doi.org/10.3390/nu50412188

7. Shatwan I.M., Winther K.H., Ellahi B., Elwood P., Ben-Shlomo Y., Givens I., et al. Association of apolipoprotein E gene polymorphisms with blood lipids and their interaction with dietary factors. Lipids Health Dis. 2018; 17(1): 98. https://doi.org/10.1186/s12944-018-0744-2

8. Semaev S., Shakhtshneider E., Shcherbakova L., Ivanoshchuk D., Orlov P., Malyutina S., et al. Associations of APOE gene variants rs429358 and rs7412 with parameters of the blood lipid profile and the risk of myocardial infarction and death in a white population of Western Siberia. Curr. Issues Mol. Biol. 2022; 44(4): 1713–24. https://doi.org/10.3390/cimb44040118

9. Rahman N., Zakiullah Z., Jan A., Saeed M., Khan M.A., Parveen Z., Iqbal J., et al. Association of APOE (rs429358 and rs7412) and PON1 (Q192R and L55M) variants with myocardial infarction in the pashtun ethnic population of Khyber Pakhtunkhwa, Pakistan. Genes (Basel). 2023; 14(3): 687. https://doi.org/10.3390/genes14030687

10. Wu H., Huang Q., Yu Z., Wu H., Zhong Z. The SNPs rs429358 and rs7412 of APOE gene are association with cerebral infarction but not SNPs rs2306283 and rs4149056 of SLCO1B1 gene in southern Chinese Hakka population. Lipids Health Dis. 2020; 19(1): 202. https://doi.org/10.1186/s12944-020-01379-4

11. Egert S., Rimbach G., Huebbe P. ApoE genotype: from geographic distribution to function and responsiveness to dietary factors. Proc. Nutr. Soc. 2012; 71(3): 410–24. https://doi.org/10.1017/S0029665112000249

12. Li W., Liu F., Liu R., Zhou X., Li G., Xiao S. APOE E4 is associated with hyperlipidemia and obesity in elderly schizophrenic patients. Sci. Rep. 2021; 11(1): 14818. https://doi.org/10.1038/s41598-021-94381-4

13. Ivanova T.I., Poluektova M.V., Khorokhorina V.A., Vorobeva O.A., Chirkova T.V., Mkrtchyan L.S., et al. Influence of APOE genotypes on biochemical indices of iron and lipid metabolism in patients with the endometrium neoplastic diseases and healthy women. Issledovaniya i praktika v meditsine. 2014; 1(1): 35–44. https://elibrary.ru/touqdh (in Russian)

14. Wang C., Ji X., Tang Z., Zhang Z., Gu X., Fang X. Combined homocysteine and apoE rs429358 and rs7412 polymorphism in association with serum lipid levels and cognition in Chinese community-dwelling older adults. BMC Psychiatry. 2022; 22(1): 223. https://doi.org/10.1186/s12888-022-03877-4

15. Zhen J., Huang X., Van Halm-Lutterodt N., Dong S., Ma W., Xiao R., et al. ApoE rs429358 and rs7412 polymorphism and gender differences of serum lipid profile and cognition in aging Chinese population. Front. Aging Neurosci. 2017; 9: 248. https://doi.org/10.3389/fnagi.2017.00248

16. Smalinskiene A., Petkeviciene J., Luksiene D., Jureniene K., Klumbiene J., Lesauskaite V. Association between APOE, SCARB1, PPARα polymorphisms and serum lipids in a population of Lithuanian adults. Lipids Health Dis. 2013; 12: 120. https://doi.org/10.1186/1476-511X-12-120

17. Mahley R.W. Central nervous system lipoproteins: ApoE and regulation of cholesterol metabolism. Arterioscler Thromb. Vasc. Biol. 2016; 36(7): 1305–15. https://doi.org/10.1161/ATVBAHA.116.307023

18. Gustavsson J., Mehlig K., Leander K., Strandhagen E., Björck L., Thelle D.S., et al. Interaction of apolipoprotein E genotype with smoking and physical inactivity on coronary heart disease risk in men and women. Atherosclerosis. 2012; 220(2): 486–92. https://doi.org/10.1016/j.atherosclerosis.2011.10.011

19. Bessueille L., Kawtharany L., Quillard T., Goettsch C., Briolay A., Taraconat N., et al. Inhibition of alkaline phosphatase impairs dyslipidemia and protects mice from atherosclerosis. Transl. Res. 2023; 251: 2–13. https://doi.org/10.1016/j.trsl.2022.06.010

20. Derradi R., Bolean M., Simão A.M.S., Caseli L., Millán J.L., Bottini M., et al. Cholesterol regulates the incorporation and catalytic activity of tissue-nonspecific alkaline phosphatase in DPPC monolayers. Langmuir. 2019; 35(47): 15232–41. https://doi.org/10.1021/acs.langmuir.9b02590

21. Cui S., Li W., Lv X., Wang P., Gao Y., Huang G. Folic acid supplementation delays atherosclerotic lesion development by modulating MCP1 and VEGF DNA methylation levels in vivo and in vitro. Int. J. Mol. Sci. 2017; 18(5): 990. https://doi.org/10.3390/ijms18050990

22. Li G., Wu X.W., Lu W.H., Ai R., Chen F., Tang Z.Z. Effect of atorvastatin on the expression of gamma-glutamyl transferase in aortic atherosclerotic plaques of apolipoprotein E-knockout mice. BMC Cardiovasc. Disord. 2014; 14: 145. https://doi.org/10.1186/1471-2261-14-145

23. Ikonnikova K.A., Eroshchenko N.N., Drozdov V.N., Shikh E.V., Serebrova S.Yu. Debating capabilities of biochemical markers of liver function in patients with alcoholic liver cirrhosis. Meditsinskii sovet. 2022; 16(7): 76–83. https://doi.org/10.21518/2079-701X-2022-16-7-76-83 https://elibrary.ru/oxixej (in Russian)