Influence of model pollutants contained in drinking water on the metabolic activity of the human duodenal adenocarcinoma cell line HuTu-80

Introduction. Currently, a promising direction in water sample toxicity research involves conducting screening analysis on human cell lines.

The purpose of this study is to assess the impact of model drinking water pollutant on the metabolic activity of the human duodenal adenocarcinoma cell line HuTu-80 to justify its further use as a test object for evaluation the toxicity of drinking water samples.

Materials and methods. The research object is the standard culture of the duodenal adenocarcinoma cell line HuTu-80. To assess the toxic effect of chloride, sulfate, and nitrate ions on the cell culture, their sodium salts were used: NaCl in concentrations ranging from 100 to 10000 mg/L, Na₂SO₄ from 20 to 2000 mg/L, and NaNO₃ from 2 to 200 mg/L. The toxic effects of pollutants on the HuTu-80 cell culture were determined using the MTT assay. Statistical analysis of the data was conducted using the Statistica 10 software (Statsoft, USA).

Results. The study evaluating the influence of model drinking water pollutants on the metabolic activity of the HuTu-80 duodenal adenocarcinoma cell culture revealed a dose-dependent effect of chloride, sulfate, and nitrate ions, resulting in a decrease in their metabolic activity.

Limitations. The application of this research method is feasible only in a specially equipped laboratory with qualified personnel.

Conclusion. The data obtained from this study may serve as a basis for the development of a test system utilizing the human intestinal cell culture to assess the toxicity of drinking water samples.

Compliance with ethical standards. The study does not require a conclusion of the Biomedical Ethics Committee.

Contribution:
Mamonova I.A. – concept and design of the study, writing the text, responsibility for the integrity of all parts of the article;
Kuzyanov D.A., Kosheleva I.S. – collection and processing of material, writing text, editing;
Erdniev L.P. – concept and design of the study, writing the text;
Evzyukova A.S. – collection and processing of material;
Shirokov A.A. – writing the text, editing;
Gusev Yu.S. – concept and design of the study;
Mikerov A.N. – approval and edition of the final version of the article.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

Received: June17, 2024 / Accepted: December 3, 2024 / Published: March 31, 2025

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