Идентификация плесневых грибов методом MALDI-TOF: подходы и перспективы (обзор литературы)

В обзоре систематизированы представленные в литературе сведения об адаптации метода MALDI-TOF к идентификации плесневых грибов. Масс-спектрометрическая идентификация бактерий методом MALDI-TOF является «золотым стандартом» в современной лабораторной диагностике. Точность, скорость, простота и невысокая стоимость одного анализа обеспечивают всё более широкое распространение этого метода. Однако применение технологии MALDI-TOF к другим микроорганизмам, в том числе плесневым грибам, затруднено, поскольку прочная стенка клеток грибного мицелия препятствует клеточному лизису и ионизации белков, а разнообразие клеток в культуре (гифы, споры, органы размножения) приводит к разнообразию ионов белков и усложняет получение единого воспроизводимого спектра для изолята. Для преодоления этих трудностей применяются различные методы культивирования, подготовки образцов, получения и обработки спектров. На рынке присутствуют готовые решения в этой области в виде коммерческих систем, однако и они требуют доработки.

Участие авторов:
Курбатова И.В. – концепция обзора, редактирование;
Ракитина Д.В. – подбор литературы, написание текста.
Все соавторы – утверждение окончательного варианта статьи и ответственность за целостность всех её частей.

Конфликт интересов. Авторы декларируют отсутствие явных и потенциальных конфликтов интересов в связи с публикацией данной статьи.

Финансирование. Исследование проведено в рамках НИР по теме «Разработка унифицированных методов, включающих отбор 8807 проб, для осуществления определения микробиологического и паразитологического загрязнения сточных вод» (шифр «Сточные воды»).

Поступила: 27.08.2024 / Принята к печати: 03.12.2024 / Опубликована: 30.04.2025

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