Antimicrobial effectiveness of gaseous ozone in the treatment of dental caries

Introduction. According to many domestic and foreign researchers, the generally accepted antibiotic preparation of carious cavities does not guarantee bacterial “cleanliness” of the operating field. The main reasons for the ineffective treatment of caries are a decrease in the effectiveness of antimicrobial agents of a wide spectrum, the imperfection of methods for caries treatment, as a result of which open, drained foci of infection become closed, with the creation of the effect of a “thermostat”. In addition, the identity of the flora of carious cavities, dentin of root canals and foci of acute odontogenic infection has now been proven.

Material and methods. 50 patients with various nosological forms of caries were examined and sanitized. In addition, in this contingent, there was carried out a microbiological study of the dentin caries cavities before preparation, after standard antiseptic treatment and subsequent treatment with gaseous ozone with the Prozone apparatus.

Results. The data obtained during the study confirm gaseous ozone to have a pronounced bactericidal effect in relation to most species of microflora found. Anaerobic microorganisms possess the greatest resistance, however, with further sowing and isolation of a pure culture, their growth was not detected, which indicates their extremely low virulence. The evaluation criterion was the growth of microorganisms in the 2nd sector according to Goild, since in this state these microorganisms are still capable of vegetating, and serve as the etiological factor of unsatisfactory treatment which manifests itself in the form of microbial demineralization of the enamel-composite compound.

1. Blohina A.A. Varianty resheniya aktual’noj problemy vosstanovleniya polostej v bokovyh zubah. DentArt. 2012; (1): 52-7.

2. Hel’vig EH., Klimek J., Attin T. Terapevticheskaya stomatologiya. Ed. prof. Politun A.M., prof. Smolyar N.I. L’vov; Gal Dent. 1999. 409 p.

3. SHumilovich B.R. Kliniko-mikrobiologicheskie izmeneniya dentina karioznyh polostej na ehtapah lecheniya kariesa i nekotoryh ego oslozhnenij: dis. … kand. med. Nauk. Voronezh, 1996. 121 p.

4. Shumilovich B.R., Kunin D.A., Krasavin V.N. Sovremennye predstavleniya ob ul’trastrukture ehmali. Problema kraevogo prileganiya kompozitov. Dental YUg. 2013; 113 (5): 24-6.

5. Shumilovich B.R., Naumova A.V., Holodovich O.V. Kliniko-laboratornoe issledovanie antimikrobnoj aktivnosti gazoobraznogo ozona pri lechenii oslozhnennogo kariesa. Maehstro stomatologii. 2011; 41 (1): 63-9.

6. Shumilovich B.R. Sovremennye aspekty resheniya problemy bakterial’noj obsemenyonnosti razlichnyh sostavlyayushchih stomatologicheskogo priyoma / SHumilovich B.R., Kosolapov V.P., Rostovtsev V.V., Filippova Z.A. Gigiena i sanitariya [Hygiene and Sanitation, Russian journal]. 2018; 97 (8): 743-9.

7. Effect of experience on quality of canal preparation with rotary nickel-titanium files. Oral. Surg.: Oral Med Radiol Endod, 1999. (in press).

8. Van Dijken J. W. Durability of resin composite restorations in high C factor cavities: a 12-year follow-up. J. Dent. 2010 Jun; 38(6):469_74. Epub 2010 Mar 1.

9. Bulaceva Z.A., Hetagurov S.K. Izuchenie kariesrezistentnogo dejstviya medicinskogo ozona. EHlektronnyj nauchno-obrazovatel’nyj vestnik «Zdorov’e i obrazovanie v XXI veke». 2008; 10 (2): 92-3.

10. Bezrukova I. V., Grudyanov A. I. Ispol’zovanie medicinskogo ozona v stomatologii. Stomatologiya. 2001; (2): 61-3.

11. Bezrukova I. V., Petruhina N. B. Ozonoterapiya v parodontologicheskoj praktike. M., 2008. 88 p.

12. Shumilovich B.R., Besehk M., Rostovcev V.V., Buryh R.I., Filippova Z.A. Kliniko-laboratornaya harakteristika sostoyaniya ehmalevo-kompozitnogo soedineniya pri primenenii individual’nyh sredstv gigieny polosti rta, soderzhashchih Brushit. ZHurnal anatomii i gistopatologii. 2018; 7 (3): 92-9.

13. Grudyanov A. I., Zorina O. A., Kulakov A. A. Kolichestvennaya ocenka mikrobiocinozov polosti rta pri zabolevaniyah parodonta. Parodontologiya. 2011; 16 (2): 18-22.

14. Dmitrieva L. A. Parodontologiya: nacional’noe rukovodstvo. Moscow, 2013. 704 p.

15. Maslennikov O. V., Kontorshchikova K. N., Gribkova I. A. Rukovodstvo po ozonoterapii. N. Novgorod, 2008. 326 p.

16. Richard Dzh. Lamont, Robert A. Berne, Merilin S. Lantc, Donal’d Dzh. Leblank Mikrobiologiya i immunologiya dlya stomatologov. Moscow, 2010. 502 p.

17. Baysan A., Lynch E. Clinical reversal of root caries using ozone: 6-month results. Am J Dent. 2007. Aug. 4 (20): 203-8.

18. Shumilovich B.R., Rostovtsev V.V., Adunts L.M., Fonstein A.M., Stanislavchuk E.S. Bio-active materials for root canal obturation the filling system with cold free-flow gutta-percha gutta flowbioseal (clinical cases report). Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2018; 9 (1): 698-707.

19. Bocci V. A. Scientific and medical aspects of ozone therapy. State of the Art. Archives of Medical Research. 2006; 37 (4): 425-35.

20. Nadeev A.D., Zinchenko V.P., Avdonin P.V., Goncharov N.V. Toksicheskie i signal’nye svojstva aktivnyh form kisloroda. Toksik. Vestnik. 2014; 15 (2): 22-7.

21. Butyugin, I.A., Volchegorskij I.A. Sostoyanie sistemy «perekisnoe okislenie lipidov - antioksidantnaya zashchita» v smeshannoj slyune u bol’nyh hronicheskim generalizovannym parodontitom. Klin. lab. diag. 2014; (2): 44-7.

22. Volchegorskij I.A., Kornilova N.V., Butyugin I.A. Sravnitel’nyj analiz sostoyaniya sistemy «perekisnoe okislenie lipidov - antioksidantnaya zashchita» v slyune bol’nyh hronicheskim parodontom lyogkoj i srednej tyazhesti. Stomatologiya. 2010; 89 (6): 24-7.

23. Grudyanov A.I., Tkacheva O.N., Avraamova T.V., Hvatova N.T. Vopros vzaimosvyazi vospalitel’nyh zabolevanij parodonta i serdechno-sosudistoj patologii. Stomatologiya. 2015; (3): 500-56.

24. Kabirova M.F. Sostoyanie svobodnoradikal’nogo okisleniya rotovoj zhidkosti u rabochih neftekhimicheskogo proizvodstva. EHkologiya i zdorov’e. 2011; (1): 76-8.

25. Bascones-Martines P., Matesanz-Perez, Escribano-Bermejo M.A., Gonales-Moles J.P. [et al.]. Periodontal disease and diabetes-Review of the Literature. Med Oral Patol Oral Cir Bucal, 2011, pp. 18-24.

26. Zubareva T.M., Komlichenko Eh.V. [et al.]. Lechenie i profilaktika. 2013. 6 (2): 117-24.

27. Shakirov D.F., Kamilov R.F., Samsonov V.M. Svobodnoradikal’noe okislenie kak zveno adaptacii organizma k faktoram proizvodstvennoj sredy. Med. biohimiya i klin. lab. diag. v aspekte modernizacii sistemy nauchnyh issledovanij: mat. Vseross. nauchno-prakt. konf. biohimikov i spec. po lab. diag. Omsk, 2011: 129-34.

28. Kolesnikova L.I., Osipova E.V., Grebenkova L.A., Vlasov B.Ya. Okislitel’nyj stress kak induktor patologicheskih narushenij v organizme cheloveka. Byul. VSNC SO RAMN. 2009; (2): 48-9.

29. Huige L., Ning X., Ulric F. Cardiovascular effects and molecular targets of resveratrol. Nitric Oxide, 2012; 26: 102-10.

30. Kolesnikova L.I., Semyonova N.V., Grebenkova L.A., Darenskaya M.A., et al. Integral’nyj pokazatel’ ocenki okislitel’nogo stressa v krovi cheloveka. Byul. ehksper. biol. i med. 2014; 157 (6): 680-3.

31. Dmitrieva L.A., Nemeryuk D.A., Gerasimova E.V. Sochetannoe ispol’zovanie sistemnoj ehnzimoterapii i antioksidantov pri lechenii zabolevanij parodonta i slizistoj obolochki rta. Stomatologiya. 2015; (2): 69-72.

32. Chapple I.L., Brock G.R., Milward M.R. et al. Compromised GCF total antioxidant capacity in periodontitis: cause or effect. J. Clin. Periodontol, 2007; 34 (2): 103-10.

33. Chapple I.L. Oxidative stress, nutrition and neutrogenomics in periodontal health and disease. Int.J. Dent. Hyg, 2006; 4 (1):15-21.